MTS - Modified Tyrodes Solution (1000 ml) - 20x concentrate
LRE-S-LSG-1000-2Modified Tyrode's Solution for use with mammalian heart tissue.
Product information
- 20x concentrate MTS solution
- Carbogen (95% oxygen and 5% carbon dioxide) supply is necessary for proper Bicarbonate pH buffering.
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Instructions for use:
20x concentrate:
For 1000 ml MTS Solution:
- Pour 50 ml Base A (20x) into a flask and add 900 ml deionized water
- Add slowly 50 ml Base B (20x)
- Oxygenate ACSF Solution during use
pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)
Use immediately once opened. Store at 4 - 8 °C
Ional composition of MTS Solution (Base A + Base B)
NaCl = 126,7 mM
KCl = 5,4 mM
CaCl2 = 1,8 mM
MgCl2 = 1,05 mM
NaH2PO4 = 0,42 mM
NaHCO3 = 22 mM
Glucose = 5 mM
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