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MH-BSCS - Modified HEPES Brain Slice Cutting Solution (1000 ml)

MH-BSCS - Modified HEPES Brain Slice Cutting Solution (1000 ml)

LRE-S-LSG-1053
  • $ 4600

Modified HEPES Brain Slice (1000 ml) (MH-BSCS) is used for cutting, recovery and storage of acute brain slices from rats and mice.

 

Product information

  • Ready to Use MH-BSCS solution

  • autoclaved

Instructions for use:

Ready to use:

For 1000 ml MH-BSCS Solution:

  1. Oxygenate 500 ml Base A for 10 min
  2. Add slowly 500 ml Base B
  3. Oxygenate MH-BSCS Solution during use

pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)

Use immediately once opened. Store at 4 - 8 °C

 

Ional composition of MH-BSCS Solution

NaCl = 92 mM

NaHCO3 = 30 mM

Glucose = 25 mM

Na L-Ascorbate = 5 mM

MgSO4 = 10 mM

NaH2PO4 = 1,25 mM

KCl = 2,5 mM

CaCl2 = 0,5 mM

HEPES = 20 mM

Thiourea = 2 mM

Na Pyruvate = 3 mM

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